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Fluorescence spectroscopy - Wikipedia

Fluorescence spectroscopy (also known as fluorimetry or spectrofluorometry) is a type of electromagnetic spectroscopy that analyzes fluorescence from a sample. It involves using a beam of light, usually ultraviolet light, that excites the electrons in molecules of certain compounds and causes them to emit light; typically, but not necessarily, visible light.

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Fluorescence imaging - Wikipedia

Fluorescence imaging is a type of non-invasive imaging technique that can help visualize biological processes taking place in a living organism. Images can be produced from a variety of methods including: microscopy, imaging probes, and spectroscopy. Fluorescence itself, is a form of luminescence that results from matter emitting light of a certain wavelength after absorbing electromagnetic ...

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Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation.It is a form of luminescence.In most cases, the emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation.The most striking example of fluorescence occurs when the absorbed radiation is in the ultraviolet region of the spectrum, and thus invisible ...

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Fluorescence microscope - Wikipedia

A fluorescence microscope is an optical microscope that uses fluorescence instead of, or in addition to, scattering, reflection, and attenuation or absorption, to study the properties of organic or inorganic substances. "Fluorescence microscope" refers to any microscope that uses fluorescence to generate an image, whether it is a more simple set up like an epifluorescence microscope or a more ...

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fluorescence energy means that replacing a fluorescent acceptor like TAMRA with a quencher such as Iowa Black TM FQ will result in an oligonucleotide construct that has no measurable fluorescence as long as the oligonucleotide tether remains intact.

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Fluorescein - an overview | ScienceDirect Topics

The fluorescent area (large arrow) had greater tumor cell density than the region with no fluorescence (small arrow). Postoperative MRI confirming complete tumor resection (H–J). According to MRI data, GTR was achieved in 78.85% (179/227) of spinal gliomas, STR in 13.22% (30/227), and PR in 7.93% (18/227). The mean clinical follow-up was 20 ± 24 months. At the final clinical follow-up, the ...

Fluorescence Fundamentals | Thermo Fisher Scientific - DE

Fluorescence intensity is quantitatively dependent on the same parameters as absorbance—defined by the Beer–Lambert law as the product of the molar extinction coefficient, optical path length and solute concentration—as well as on the fluorescence quantum yield of the dye and the excitation source intensity and fluorescence collection efficiency of the instrument (Table 1). In dilute ...

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Fluorescein und andere Phthaleine wurden 1871 von Adolf von Baeyer entdeckt. Den alternativen Namen Resorcinphthalein verdankt der Stoff den Reaktanten, aus denen ihn Adolf von Baeyer synthetisierte.Er mischte Phthalsäureanhydrid (auch Phthalsäure ist verwendbar) mit Resorcin und fügte konzentrierte Schwefelsäure als wasserabspaltendes Mittel hinzu.

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Fluorescence emission behaves in a similar way: the fluorescence output of a fluorophore is most likely to occur at a particular wavelength. This wavelength is the emission maximum for that fluorophore. The excited fluorophore can also emit light at wavelengths near the emission maximum, as shown. However, this light will be less intense. Emission range and maximum. A) Emission spectrum (line ...

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Fluorescence Microscopy To understand how fluorescence microscopy works and why it has become so important to modern biology, one must understand what the term fluorescence means. Fluorescence is the luminescence of a substance when it is excited by radiation. In microscopy, fluorescence is used as a means of preparing specific biological probes. Some biological substances like chlorophyll and ...

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Fluorescence is the most rapidly expanding microscopy technique in both the medical and biological sciences, a fact which has spurred the development of more sophisticated microscopes and fluorescence accessories. Fluorescence is the most rapidly expanding microscopy technique in both the medical and biological sciences, a fact which has spurred the development of more sophisticated ...

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Fluorescence from the vessel or substrate upon which the cells are grown; The medium used during imaging can contribute to background fluorescence; Figure 1. Background fluorescence can arise from many sources in your experiment. What you can do to decrease background fluorescence. Getting the best data from your fluorescent image requires you to do everything you can to make the difference ...

Fluorescence Analysis - an overview | ScienceDirect Topics

Fluorescence assays on nasal wash specimens are the diagnostic test of choice because most of these pathogens are concentrated in the nasopharynx. The sensitivity of indirect immunofluorescence assays is greater than 90% to 95% sensitive for RSV; parainfluenza 1, 2, 3; and influenza A and B. A rapid immunoassay for RSV and influenza is also available. Adenovirus immunofluorescence assays are ...

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This process is called fluorescence. To “fluoresce” means to emit light via this process. A fluorophore is a molecule that is capable of fluorescing. Some examples of fluorescent molecules include Invitrogen™ Alexa Fluor™ dyes, structural stains like DAPI or Invitrogen™ MitoTracker™ Red, and green fluorescent protein. Ground state In its ground state, the fluorophore molecule is in ...

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Fluorescence spectroscopy and related techniques have become important tools for the analytical chemist, particularly in drug analysis and the measurement of environmental pollutants. Although fluorescence spectrometers have been commercially available for nearly thirty years, the past decade has seen a great diversification of applications as well as a deeper theoretical understanding of ...

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Fluorescence Lifetime Sensing: Most of the fluorescence sensors and assays that are in use today are based on intensity measurements. Though these methods are easier to implement they lack robustness and they require frequent calibration [6]. Many difficulties that are associated with intensity-based measurements can be circumvented using lifetime-based measurements. Lifetime-based ...

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fluorescence anisotropy measurements: Last post 19 Apr 07, 11:45: the mixing behavior of DPPC with PFOS was studied using fluorescence anisotropy measurements… 1 Replies: Fluroszenz: Last post 20 Mar 08, 16:11: Mit Hilfe eine Lasers kann man eine Fluroszenz der Probe erzeugen Fluroszenz in Sinne von L… 2 Replies: Röntgenfluoreszensanalysator: Last post 28 Sep 05, 20:37: Ist wohl ein ...

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Fluorescence microscopy with incident (reflected light or episcopic) illumination was first developed in the late 1920s to observe fluorescence emission in opaque metallurgical specimens. Like their brightfield counterparts, these reflected light fluorescence instruments employed half-mirror beamsplitters that are restricted to an overall efficiency of approximately 25 percent (after losing 50 ...

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